Bone regeneration is a very complex, but well-orchestrated continuous remodelling process of bone formation. The entire adult life witnesses, bone as a tissue which possesses a tremendous inherent capacity for regeneration through the coordinated biological activity of different cells. However, in case of large defects, which arises from fractures due to accidents or disease conditions, where the injury goes beyond a critical limit, the bone will not be able to self-heal without surgical and therapeutic intervention. A variety of currently available bone grafts are being used to replace the missing bone but they suffer from one or the other limitations such as poor graft integration, restricted graft availability. Through this project, the aim is to develop an implant material, using exosomes from the human donor’s bone cells, which will augment dental bone regeneration. From our R&D, we have established that exosomes isolated from mineralizing osteoblasts have the potential to elevate the osteogenesis in vitro. We have developed cross-linked polymeric scaffolds that provide mechanical strength and an extracellular matrix for holding and releasing an appropriate dose of exosomes at the target site. This bone regeneration method using exosome-loaded polymeric scaffold has been proved effective in preclinical studies involving critical-sized calvarial defects